What We Do

LifeEDIT uses a proprietary bioinformatic pipeline to mine a rich microbial genome sequence database for novel gene editing systems. Prioritized systems are characterized and deployed in gene editing applications both in-house and through research partnerships in human therapeutics and agriculture.

LifeEDIT has bioinformatically identified 1000’s of novel CRISPR-Cas gene editing systems in AgBiome’s microbial genome sequence database. This includes >800 Class II systems with single effector proteins. All of these come from non-pathogenic bacteria and many are only distantly related to systems in common use. LifeEDIT has established a pipeline for further characterization of prioritized systems. Guide RNA determination is facilitated by RNA-seq analysis of the cognate microbial isolate. PAM requirements are determined through library-based assays. New editing systems are validated for use in eukaryotes using mammalian and plant cell-based assays. Additional characterization including efficiency and off-target information is obtained through in vitro and in vivo assays. The end result is an ever-growing collection of novel gene editing tools with reduced immune response risk, and diverse in sequence, size, PAM requirements, fidelity, and efficiency. These new systems are deployed for gene editing applications in human therapeutics, agriculture, and industrial biotechnology in-house and through research partnerships.

Bioinformatics pipeline for novel genome editing tool discovery

 We perform an homology search with permissive criteria against curated reference databases

All Hits

(filter for false positives)

High Quality Hits

(search vs. known sequences)

OUTPUT:

System type and Subtype System Completeness Novelty vs. Known
Relationship to Patents Isolate Taxonomy and Source Sequence and Region